The SDF1 level of FA7DOWN cells was decreased by 24

The SDF1 level of FA7DOWN cells was decreased by 24.76% (cDNA, in vitro) and 34.17% (protein, in vitro) compared to that in BCX 1470 methanesulfonate FSHUS cells (lower or 2.39 times (protein, in vitro) higher than those in FSHUS and PNCEV cells (Transcriptome sequencing heat maps (A1, A2) and cDNA expression (A3) of SDF1/CXCR4 in FA7DOWN/FSHUS and PA7UP/PNCEV cells. higher than those in P cells with low lymphatic metastatic potentialExpression of SDF1 was higher than that of CXCR4 in P cells and normal liver cells, while expression of CXCR4 was higher than that of SDF1 in F cells. Expression levels of SDF1/CXCR4 were completely consistent with AnnexinA7 regulation. After the AnnexinA7 gene was downregulated or upregulated, expression levels of SDF1/CXCR4 in FA7DOWN/PA7UP cells were lower or higher than those in FSHUS/PNCEV BCX 1470 methanesulfonate cellsFurthermore, CXCR4 was more sensitively modulated by AnnexinA7 regulation than SDF1. Conclusions High co-expression of SDF1/CXCR4 is a molecular characteristic of hepatocarcinoma cells, especially those with high lymphatic metastatic potential. AnnexinA7 positively regulates expression levels of SDF1/CXCR4, in particular CXCR4, and AnnexinA7 is a functional regulator of SDF1/CXCR4mRNA regulation efficiency of AnnexinA7 BCX 1470 methanesulfonate in vitro (B). Western blot results and protein regulation efficiency of AnnexinA7 in vitro (C) Open in a separate window Fig. 4 Expression of SDF1 and CXCR4 in different hepatocarcinoma cells and normal liver cells in vitro and in vivo.Cytoimmunofluorescence cell nuclear DAPI staining (A1), cell staining (A2), merged picture(A) and immunohistochemistry (C)] analysis of SDF1 (Left) and CXCR4 (Right) expression in normal hepatocytes, F/P cells in vitro (A1, A2, A) and in vivo (C). SDF1 expression in vitro (A1, A2, A, Left). CXCR4 expression in vitro (A1, A2, A, Right). SDF1 expression in vivo (C, Left). CXCR4 expression in vivo (C, Right). Cytoimmunofluorescence and immunohistochemistry OD values for SDF1/CXCR4 in normal hepatocytes and F/P cells in vitro (B) and in vivo (D). * indicates and 1.35 times (mRNA, in vivohigher than those in P cells (in vivo Transcriptome sequencing, qRT-PCR, Western blotting, cytoimmunofluorescence and immunohistochemistry showed that SDF1 was mainly localized in the cytoplasm of cells, and in a small amount, it was located in the cell membrane; while CXCR4 was mainly localized in the cell membrane, and in a small amount, it was localized in the cytoplasm in F/P, FA7DOWN/PA7UP and FSHUS/PNCEV cells both in vitro and in vivo (Fig. 6A, B, C, D). More importantly, there was a significant positive relationship between the expression of SDF1/CXCR4 and AnnexinA7 gene BCX 1470 methanesulfonate regulation. The downregulation or upregulation of AnnexinA7 resulted in decreased or increased expression of SDF1/CXCR4, respectively, showing a highly homotropic pattern. After downregulation of AnnexinA7, the expression levels of SDF1/CXCR4 in FA7DOWN cells were lower than those in FSHUS and F cells in vitro and in vivo. The SDF1 level of FA7DOWN cells was decreased by 24.76% (cDNA, in vitro) and 34.17% (protein, in vitro) compared to that in FSHUS cells (lower or 2.39 times (protein, in vitro) higher than those in FSHUS and PNCEV cells (Transcriptome sequencing heat maps (A1, A2) and cDNA expression (A3) of SDF1/CXCR4 in FA7DOWN/FSHUS and PA7UP/PNCEV cells. qRT-PCR (B1, B2) and Western blot (C1, C2) analysis of SDF1/CXCR4 mRNA and protein expression respectively, in FA7DOWN, FSHUS, PA7UP, and PNCEV cells in vitro (Left) and in vivo (Right)mRNA expression in FA7DOWN and FSHUS cells (B1, Left) as well as in PA7UP/PNCEV cells (B2, Left) in vitro. mRNA expressions in FA7DOWN and FSHUS cells (B1, Right) as well as in PA7UP/PNCEV cells (B2, right) in vivo. KGFR Protein expressions in FA7DOWN and FSHUS cells (C1, Left) as well as in PA7UP, PNCEV cells (C2, Left) in vitro. Protein expressions in FA7DOWN and FSHUS cells (C1, Right) as well as in PA7UP and PNCEV cells (C2, Right) in vivo. mRNA results between different groups in vitro and in vivo (E). Western blot OD densities of SDF1/CXCR4 in FSHUS and FA7DOWN cells (D1, Left) as well as in PA7UP and PNCEV cells which favor cell proliferation and metastasis of tumor cells. Furthermore, the expression levels of SDF1/CXCR4 in F cells with high lymphatic metastatic.